Human rhinoviruses (HRVs) are single-stranded RNA viruses belonging to the Enterovirus genus of the Picornaviridae family that frequently cause mild upper respiratory tract infections (“colds”). Over 100 serotypes of rhinovirus have been identified. Transmission of HRV occurs most frequently via person-to-person contact and through airborne transmission. Although different therapeutic approaches to inhibit HRV replication have been tested in the clinic, some of which demonstrated modest effects on the virus, currently, there are no approved drugs for the treatment of rhinovirus infection. Infection with rhinoviruses has also been shown to be an important contributor of exacerbations of chronic obstructive pulmonary disease (COPD) and asthma.